Difference between revisions of "Utah Genome Project"

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== Utah Genome Project ==
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The [http://healthsciences.utah.edu/utah-genome-project/ Utah Genome Project] has developed a detailed variant calling protocol based on the [https://www.broadinstitute.org/gatk/ GATK HapoltypeCaller].  This protocol very closely follows the [https://www.broadinstitute.org/gatk/guide/best-practices GATK Best Practices] for variant calling on DNAseq data, however here we provide detailed and versioned protocol that includes exact command lines for each step in the analysis as well as links to accessory datasets.
*The main Project [http://healthsciences.utah.edu/utah-genome-project/ site]
 
  
== Variant Analysis Pipeline Development ==
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== UGP Variant Calling ==
  
* [[UGP_Variant_Pipeline | UGP Variant Pipeline]]
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* [[UGP_Variant_Protocol | UGP Variant Calling Protocol]]
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* [http://srynobio.github.io/UGPp UGPp] - a lightweight NGS analysis pipeline implementing the UGP variant calling protocol.
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* The [http://healthcare.utah.edu/huntsmancancerinstitute/research/shared-resources/center-managed/bioinformatics/ HCI Informatics] team have created a pipeline using [http://healthcare.utah.edu/huntsmancancerinstitute/research/shared-resources/center-managed/bioinformatics/useful-links/software-tomato2.php Tomato2] that implements the UGP Variant Protocol
  
== UGP Pipeline ==
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== Protocol Development ==
  
 
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The UGP and the [http://healthsciences.utah.edu/utah-genome-project/family/2014/050614-USTAR%20Center%20for%20Genetic%20Discovery%20Launches.php UCGD] are developing a variant protocol that utilizes the [https://github.com/ekg/freebayes FreeBayes] variant caller from the [http://www.bioscience.utah.edu/faculty/molecular-biology-faculty/marth/ Marth Lab].  We are in the process of optimizing this protocol and benchmarking its performance on various datasets.
*[http://srynobio.github.io/cApTUrE cApTUrE] is a lightweight NGS pipeline, created for the
 Utah Genome Project (UGP)
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* [[FreeBayes_Variant_Protocol | FreeBayes Variant Protocol]]

Latest revision as of 19:15, 27 June 2015

The Utah Genome Project has developed a detailed variant calling protocol based on the GATK HapoltypeCaller. This protocol very closely follows the GATK Best Practices for variant calling on DNAseq data, however here we provide detailed and versioned protocol that includes exact command lines for each step in the analysis as well as links to accessory datasets.

UGP Variant Calling

Protocol Development

The UGP and the UCGD are developing a variant protocol that utilizes the FreeBayes variant caller from the Marth Lab. We are in the process of optimizing this protocol and benchmarking its performance on various datasets.