Difference between revisions of "UGP Variant Pipeline 0.0.2"

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Line 52: Line 52:
  
 
== Alignment ==
 
== Alignment ==
 
  
 
Index the reference sequence if this has not already been done
 
Index the reference sequence if this has not already been done
  
  bwa index -a bwtsw reference_genome.fa
+
  bwa index -a bwtsw human_g1K_v37.fasta
  
 
The 'bwa aln' program will find the reference coordinates of the input reads (independent of their mate-pair). The following parameters are those used by the 1KG project for aligning Illumina data.
 
The 'bwa aln' program will find the reference coordinates of the input reads (independent of their mate-pair). The following parameters are those used by the 1KG project for aligning Illumina data.
  
  bwa aln -q 15 reference.fasta file.fastq > lane1_r1.sai # One lane of reads first in pair
+
  bwa aln -q 15 human_g1K_v37.fasta file.fastq > lane1_r1.sai # One lane of reads first in pair
  bwa aln -q 15 reference.fasta file.fastq > lane1_r2.sai # One lane of reads second in pair
+
  bwa aln -q 15 human_g1K_v37.fasta file.fastq > lane1_r2.sai # One lane of reads second in pair
  
 
The 'bwa sampe'.  For paired-end reads, the maximum insert size is taken to be 3 times the expected insert size.
 
The 'bwa sampe'.  For paired-end reads, the maximum insert size is taken to be 3 times the expected insert size.
Line 93: Line 92:
 
* -N        non-iterative mode: search for all n-difference hits (slooow)
 
* -N        non-iterative mode: search for all n-difference hits (slooow)
 
* -f FILE  file to write output to instead of stdout
 
* -f FILE  file to write output to instead of stdout
 
 
  
 
The AddOrReplaceReadGroups tool will add the correct readgroup ID to the BAM files
 
The AddOrReplaceReadGroups tool will add the correct readgroup ID to the BAM files
Line 115: Line 112:
  
 
Alignment BAM files are improved in various ways to help increase the quality and speed of subsequent variant calling steps.
 
Alignment BAM files are improved in various ways to help increase the quality and speed of subsequent variant calling steps.
 +
 +
=== Sort BAM ===
 +
 +
java -jar -Xmx3g /tools/picard-tools-1.32/SortSam.jar \
 +
    INPUT=/output/filename.b37_1kg.recal.bam            \
 +
    OUTPUT=/output/filename.b37_1kg.recal.sorted.bam    \
 +
    CREATE_INDEX=TRUE                                  \
 +
    SORT_ORDER=coordinate                              \
 +
    VALIDATION_STRINGENCY=LENIENT                      \
 +
    TMP_DIR=/local
  
 
=== Merge lane level BAMs to individual ===  
 
=== Merge lane level BAMs to individual ===  
Line 129: Line 136:
 
     SORT_ORDER=coordinate        \                                                                                                                         
 
     SORT_ORDER=coordinate        \                                                                                                                         
 
     VALIDATION_STRINGENCY=SILENT
 
     VALIDATION_STRINGENCY=SILENT
 +
 +
=== Mark Duplicates ===
 +
 +
Remove PCR/Optical duplicate reads
 +
 +
java $jvm_args -jar MarkDuplicates.jar \
 +
    INPUT=lane1.bam                    \
 +
    OUTPUT=lane1_markdup                \
 +
    ASSUME_SORTED=TRUE                  \
 +
    METRICS_FILE=lane1_dup_metrics.txt  \
 +
    VALIDATION_STRINGENCY=SILENT        \
 +
    TMP_DIR=/big/drive
  
 
=== GATK Local Realignment of Indels ===
 
=== GATK Local Realignment of Indels ===
  
  java -Xmx10g -jar /usr/local/GenomeAnalysisTK-2.4-7-g5e89f01//GenomeAnalysisTK.jar                         \
+
  java -Xmx10g -jar /usr/local/GenomeAnalysisTK-2.4-7-g5e89f01//GenomeAnalysisTK.jar \
     -I all_bams.list                               \
+
     -I all_bams.list                                                 \
     -T RealignerTargetCreator                     \
+
     -T RealignerTargetCreator                                       \
     -R fasta/hs_ref_GRCh37.p10_ALL_chr_hs37d5.fa   \
+
     -R fasta/hs_ref_GRCh37.p10_ALL_chr_hs37d5.fa     \
     -o realign.intervals                             \
+
     -o lane_1.realign.intervals                       \
     -known known_indels/ALL.wgs.indels_mills_devine_hg19_leftAligned_collapsed_double_hit.indels.sites.vcf \
+
     -known Mills_and_1000G_gold_standard.indels.b37.vcf                             \
     -known known_indels/ALL.wgs.low_coverage_vqsr.20101123.indels.sites.vcf                               \
+
     -known 1000G_phase1.indels.b37.vcf.gz                                          \
     -nt 24                                                                                                 \
+
     -nt 24                                                                         \
     >  RealignerTargetCreator                                                                             \
+
     >  RealignerTargetCreator                                                       \
 
     2> RealignerTargetCreator.error &
 
     2> RealignerTargetCreator.error &
  
Line 282: Line 301:
  
 
  java $jvm_args -jar MergeSamFiles.jar INPUT=$tag_stripped_bam_file(s) OUTPUT=$library_level_bam VALIDATION_STRINGENCY=SILENT
 
  java $jvm_args -jar MergeSamFiles.jar INPUT=$tag_stripped_bam_file(s) OUTPUT=$library_level_bam VALIDATION_STRINGENCY=SILENT
 
=== Mark PCR Duplicates ===
 
 
[http://picard.sourceforge.net/ Picard] MarkDuplicates is used to mark PCR Duplicates
 
 
java $jvm_args -jar MarkDuplicates.jar INPUT=$library_level_bam OUTPUT=$markdup_bam_file ASSUME_SORTED=TRUE METRICS_FILE=/dev/null VALIDATION_STRINGENCY=SILENT
 
 
java -Xmx4g -jar /tools/picard-tools-1.32/MarkDuplicates.jar \ INPUT=/output/filename.b37_1kg.sorted.bam \ OUTPUT=/output/filename.b37_1kg.dedup.bam \ METRICS_FILE=/output/filename.b37_1kg.dedup.metrics \ REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT \ TMP_DIR=/local
 
  
 
=== Merge All BAMS for the Project ===
 
=== Merge All BAMS for the Project ===

Revision as of 16:21, 6 September 2013

Utah Genome Project

Variant Calling Pipeline 0.0.2

Sept. 2013

Data Source

Data sets used for the variant calling pipeline come from the Broad GSA (GATK) group as the 'GATK resource bundle 2.5' version 2.5

wget -r ftp://gsapubftp-anonymous@ftp.broadinstitute.org/bundle/2.5/b37
  • Reference Genome (GRCh37):
human_g1K_v37.fasta
  • High confidence SNVs
 1000G_omni2.5.b37.vcf
 hapmap_3.3.b37.vcf
  • dbSNP
 dbsnp_137.b37.vcf.gz
  • High confidence indels
 Mills_and_1000G_gold_standard.indels.b37.vcf.gz

Sequencing

This pipeline is designed for 100 bp Illumina HiSeq PE exome or WGS sequence data with Sanger/Illumina 1.9 quality encoding.

Validate File Integrity with md5sum

An md5sum signature should be provided for each FastQ file by the sequencing center. After the file has been downloaded, locally check the md5sum to be sure that no data corruption occurred during the file transfer.

md5sum file.fastq > file_local.md5
diff file_local.md5 file_provided.md5

If the md5sum signature differs from that provided for the file:

  • Check to be sure you have the correct file.
  • Check if the md5sum was calculated on that compressed or uncompressed file by the provider and be sure to do the same with the local copy.
  • Try the download again.
  • Contact the sequence provider.

FastQ File Analyses

fastqc 9958X1_130327_700179R_0404_BD1YNUACXX_7_1.txt

From the fastqc_data.txt file we check the following values:

  • Encoding (must be Sanger / Illumina 1.9)
  • Total Sequences (Need to develop a acceptable range)
  • Filtered Sequences (Should be 0 or at least very low)
  • Sequence length (must be ~ 100 bp)
  • %GC (should be 45 < x < 55)
  • Total Duplicate Percentage (This value may not be valuable - an acceptable range has not been determined).

Alignment

Index the reference sequence if this has not already been done

bwa index -a bwtsw human_g1K_v37.fasta

The 'bwa aln' program will find the reference coordinates of the input reads (independent of their mate-pair). The following parameters are those used by the 1KG project for aligning Illumina data.

bwa aln -q 15 human_g1K_v37.fasta file.fastq > lane1_r1.sai # One lane of reads first in pair
bwa aln -q 15 human_g1K_v37.fasta file.fastq > lane1_r2.sai # One lane of reads second in pair

The 'bwa sampe'. For paired-end reads, the maximum insert size is taken to be 3 times the expected insert size.

bwa sampe -P reference_genome.fa lane1_r1.sai lane1_r2.sai lane1_r1.fastq lane1_r1.fastq | samtools view -bt reference_genome.fa.fai -o lane1 -

This will switch to bwa mem soon.

bwa mem reference_genome.fa lane1_r1.fq lane2_r2.fq | samtools view -bt reference_genome.fa.fai -o lane1 -

Bwa Command Line Options:

  • -n NUM max #diff (int) or missing prob under 0.02 err rate (float) [0.04]
  • -o INT maximum number or fraction of gap opens [1]
  • -e INT maximum number of gap extensions, -1 for disabling long gaps [-1]
  • -i INT do not put an indel within INT bp towards the ends [5]
  • -d INT maximum occurrences for extending a long deletion [10]
  • -l INT seed length [32]
  • -k INT maximum differences in the seed [2]
  • -m INT maximum entries in the queue [2000000]
  • -t INT number of threads [1]
  • -M INT mismatch penalty [3]
  • -O INT gap open penalty [11]
  • -E INT gap extension penalty [4]
  • -R INT stop searching when there are >INT equally best hits [30]
  • -q INT quality threshold for read trimming down to 35bp [0]
  • -c input sequences are in the color space
  • -L log-scaled gap penalty for long deletions
  • -N non-iterative mode: search for all n-difference hits (slooow)
  • -f FILE file to write output to instead of stdout

The AddOrReplaceReadGroups tool will add the correct readgroup ID to the BAM files

nohup java -d64 -Xmx8g -jar /usr/local/picard-tools-1.88/AddOrReplaceReadGroups.jar \
   INPUT=9958X1_7_1.bam                                                            \
   OUTPUT=9958X1_RG.bam                                                            \
   CREATE_INDEX=TRUE                                                               \
   MAX_RECORDS_IN_RAM=2000000                                                      \
   VALIDATION_STRINGENCY=SILENT                                                    \
   TMP_DIR=/tmp                                                                    \
   SORT_ORDER=coordinate                                                           \
   RGID=9958X1                                                                     \
   RGLB=9958X1                                                                     \
   RGPL=illumina                                                                   \
   RGPU=1                                                                          \
   2> 9958X1_AddOrReplaceReadGroups.error &

BAM File Analyses

Alignment BAM files are improved in various ways to help increase the quality and speed of subsequent variant calling steps.

Sort BAM

java -jar -Xmx3g /tools/picard-tools-1.32/SortSam.jar \
   INPUT=/output/filename.b37_1kg.recal.bam            \
   OUTPUT=/output/filename.b37_1kg.recal.sorted.bam    \
   CREATE_INDEX=TRUE                                  \
   SORT_ORDER=coordinate                              \
   VALIDATION_STRINGENCY=LENIENT                      \
   TMP_DIR=/local

Merge lane level BAMs to individual

java -jar -Xmx4g /tools/picard-tools-1.90/MergeSamFiles.jar \                                                                                              
    INPUT=alignment.bam          \                                                                                                                         
    INPUT=alignment.bam          \                                                                                                                         
    OUTPUT=merged.bam            \                                                                                                                         
    CREATE_INDEX=TRUE            \                                                                                                                         
    ASSUME_SORTED=true           \                                                                                                                         
    USE_THREADING=true           \                                                                                                                         
    TMP_DIR=/tmp                 \                                                                                                                         
    MAX_RECORDS_IN_RAM=30000000  \                                                                                                                         
    SORT_ORDER=coordinate        \                                                                                                                         
    VALIDATION_STRINGENCY=SILENT

Mark Duplicates

Remove PCR/Optical duplicate reads

java $jvm_args -jar MarkDuplicates.jar \
   INPUT=lane1.bam                     \
   OUTPUT=lane1_markdup                \
   ASSUME_SORTED=TRUE                  \
   METRICS_FILE=lane1_dup_metrics.txt  \
   VALIDATION_STRINGENCY=SILENT        \
   TMP_DIR=/big/drive

GATK Local Realignment of Indels

java -Xmx10g -jar /usr/local/GenomeAnalysisTK-2.4-7-g5e89f01//GenomeAnalysisTK.jar \
   -I all_bams.list                             		                    \
   -T RealignerTargetCreator                    		                    \
   -R fasta/hs_ref_GRCh37.p10_ALL_chr_hs37d5.fa 				    \
   -o lane_1.realign.intervals                    				    \
   -known Mills_and_1000G_gold_standard.indels.b37.vcf                             \
   -known 1000G_phase1.indels.b37.vcf.gz                                           \
   -nt 24                                                                          \
   >  RealignerTargetCreator                                                       \
   2> RealignerTargetCreator.error &
java -Xmx10g -jar /usr/local/GenomeAnalysisTK-2.4-7-g5e89f01/GenomeAnalysisTK.jar                          \
   -T IndelRealigner                                                                                      \
   -R fasta/hs_ref_GRCh37.p10_ALL_chr_hs37d5.fa                                                           \
   -I 9958X1_RG.bam                                                                                       \
   -I 9958X2_RG.bam                                                                                       \
   -I 9958X3_RG.bam                                                                                       \
   -I 9958X4_RG.bam                                                                                       \
   -targetIntervals realign.intervals                                                                     \
   -o 9958X_Realigned.bam                                                                                 \
   -known known_indels/ALL.wgs.indels_mills_devine_hg19_leftAligned_collapsed_double_hit.indels.sites.vcf \
   -known known_indels/ALL.wgs.low_coverage_vqsr.20101123.indels.sites.vcf                                \
   >  IndelRealigner.out                                                                                  \
   2> IndelRealigner.error

Or this ??:

java -Djava.io.tmpdir=/local -Xmx8g -jar GenomeAnalysisTK.jar \
   -l INFO -T IndelRealigner \
   -U ALLOW_UNINDEXED_BAM    \
   -I /output/filename.b37_1kg.dedup.bam \
   -targetIntervals /resources//hg19/intervals/realign_intervals_hg19_b37_1kg.intervals \
   -R /resources//hg19/indices/b37_1kg.fa \
   -D /resources//hg19/dbsnp/dbsnp_129_b37_b37_1kg.rod \
   -[B:indels,VCF B:indels,VCF] /resources//hg19/indels/1kg.pilot_release.merged.indels.sites./hg19.b37_1kg.vcf \
   -o /output/filename.b37_1kg.realigned.bam
   -knownsOnly
   -LOD 0.4
   -compress 0

Known indels are from the following files:

Fix Mate Information

java -jar -Xmx6g /tools/picard-tools-1.32/FixMateInformation.jar \
   INPUT=/output/filename.b37_1kg.realigned.bam \
   OUTPUT=/output/filename.b37_1kg.matefixed.bam \
   SORT_ORDER=coordinate                       \
   VALIDATION_STRINGENCY=SILENT                \
   TMP_DIR=/local

GATK Quality Score Recalibration

java [jvm_args] -jar GenomeAnalysisTK.jar                                 \
     -T CountCovariates                                                   \
     -R $reference_fasta                                                  \
     -I $realigned_bam_file                                                \
     -recalFile recal_data.csv                                            \
     -knownSites $known_sites_file(s)                                      \
     -l INFO                                                              \
     -L '1;2;3;4;5;6;7;8;9;10;11;12;13;14;15;16;17;18;19;20;21;22;X;Y;MT' \
     -cov ReadGroupCovariate                                              \
     -cov QualityScoreCovariate                                           \
     -cov CycleCovariate                                                  \
     -cov DinucCovariate                                                  \
java [jvm_args] -jar GenomeAnalysisTK.jar \ 
     -T TableRecalibration                \
     -R $reference_fasta                  \
     -recalFile recal_data.csv            \
     -I $realigned_bam_file                \
     -o $recalibrated_bam_file             \
     -l INFO                              \
     -compress 0                          \
     -disable_bam_indexing                \


Known sites for recalibration are from dbSNP135:

Sort and index recalibrated alignment (~5h)

java -jar -Xmx3g /tools/picard-tools-1.32/SortSam.jar \
   INPUT=/output/filename.b37_1kg.recal.bam            \
   OUTPUT=/output/filename.b37_1kg.recal.sorted.bam    \
   SORT_ORDER=coordinate                              \
   VALIDATION_STRINGENCY=LENIENT                      \
   TMP_DIR=/local
java -jar -Xmx3g /tools/picard-tools-1.32/BuildBamIndex.jar \
   INPUT=/output/filename.b37_1kg.recal.sorted.bam           \
   OUTPUT=/output/filename.b37_1kg.recal.sorted.bam.bai      \
   VALIDATION_STRINGENCY=LENIENT \ TMP_DIR=/local

Calculate covariates after realignment and recalibration

java -jar -Xmx2g GenomeAnalysisTK.jar                      \
   -l INFO                                                 \
   -T CountCovariates                                      \
   -U ALLOW_UNINDEXED_BAM                                  \
   -R /resources//hg19/indices/b37_1kg.fa                  \
   --DBSNP /resources//hg19/dbsnp/dbsnp_129_b37_b37_1kg.rod \
   -I /output/filename.b37_1kg.recal.sorted.bam             \
   -cov ReadGroupcovariate                                 \
   -cov QualityScoreCovariate                              \
   -cov CycleCovariate                                     \
   -cov DinucCovariate                                     \
   -recalFile /output/filename.b37_1kg.recal.covariate_table.csv

Analyze covariates before and after

java -jar -Xmx4g AnalyzeCovariates.jar                             \
   -l INFO                                                         \
   -resources /resources//hg19/indices/b37_1kg.fa                  \
   --recal_file /output/filename.b37_1kg.matefixed.covariate_table.csv \
   -outputDir /output/filename.b37_1kg.recal.stats_before/          \
   -Rscript ${rscript}
   -ignoreQ 5
java -jar -Xmx4g AnalyzeCovariates.jar                          \
   -l INFO                                                      \
   -resources /resources//hg19/indices/b37_1kg.fa               \
   --recal_file /output/filename.b37_1kg.recal.covariate_table.csv \
   -outputDir /output/filename.b37_1kg.recal.stats_after/        \
   -Rscript ${rscript}                                          \
   -ignoreQ 5

Generate the SAM MD Tag

The MD tag in SAM files provides a string representation of mismatching positions. The CIGAR string used in SAM files does not provide full detail of mismatch positions. The samtools calmd command is used to generate MD tags as well as which fix the NM tags (Edit distance to the reference) and introduce the BQ tags (Base Alignment Quality) which can be used during variant calling.

samtools calmd -Erb recalibrated_file.bam $reference.fasta > $bq_file.bam

Strip Extraneous BAM Tags

Run-level BAMs have extraneous tags (OQ, XM, XG, XO) stripped from them, to reduce total file size by around 30%.

GATK ReduceReads ???

Merge BAMS

Merge all BAMS for a given individual

java $jvm_args -jar MergeSamFiles.jar INPUT=$tag_stripped_bam_file(s) OUTPUT=$library_level_bam VALIDATION_STRINGENCY=SILENT

Merge All BAMS for the Project

This happens above... Picard MergeSamFiles is used to Merge all the SAM files for a given project.

java $jvm_args -jar MergeSamFiles.jar INPUT=$markdup_bam_file(s) OUTPUT=$platform_level_bam VALIDATION_STRINGENCY=SILENT

BAM Quality Control

  • Picard Tools
    • BamIndexStats
    • CalculateHsMetrics ??
    • CleanSam
    • CollectAlignmentSummaryMetrics
    • CollectGcBiasMetrics
    • CollectInsertSizeMetrics
    • CollectMultipleMetrics
    • CollectTargetedPcrMetrics
    • EstimateLibraryComplexity
    • FixMateInformation
    • MarkDuplicates
    • MeanQualityByCycle
    • QualityScoreDistribution
    • ValidateSamFile
  • GATK QC
  • [1]
  • [2]

CollectAlignmentSummaryMetrics

java -jar -Xmx4g /tools/picard-tools-1.32/CollectAlignmentSummaryMetrics.jar \
   I=/output/filename.b37_1kg.sorted.bam                                      \
   O=/output/filename.b37_1kg.AlignmentSummaryMetrics                         \
   R=/resources//hg19/indices/b37_1kg.fa                                     \
   VALIDATION_STRINGENCY=LENIENT                                             \
   TMP_DIR=/local

CollectGcBiasMetrics

java -jar /tools/picard-tools-1.32/CollectGcBiasMetrics.jar \
   R=/resources//hg19/indices/b37_1kg.fa                    \
   I=/output/filename.b37_1kg.sorted.bam                     \
   O=/output/filename.b37_1kg.GcBiasMetrics                  \
   CHART=/output/filename.b37_1kg.GcBiasMetrics.pdf          \
   VALIDATION_STRINGENCY=LENIENT                            \
   TMP_DIR=/local

CollectInsertSizeMetrics

java -jar /tools/picard-tools-1.32/CollectInsertSizeMetrics.jar \
   I=/output/filename.b37_1kg.sorted.bam                         \
   O=/output/filename.b37_1kg.CollectInsertSizeMetrics           \
   H=/output/filename.b37_1kg.CollectInsertSizeMetrics.pdf       \
   VALIDATION_STRINGENCY=LENIENT                                \
   TMP_DIR=/local

MeanQualityByCycle

java -jar /tools/picard-tools-1.32/MeanQualityByCycle.jar \
   I=/output/filename.b37_1kg.sorted.bam                   \
   O=/output/filename.b37_1kg.MeanQualityByCycle           \
   CHART=/output/filename.b37_1kg.MeanQualityByCycle.pdf   \
   VALIDATION_STRINGENCY=LENIENT                          \
   TMP_DIR=/local

QualityScoreDistribution

java  -jar  /tools/picard-tools-1.32/QualityScoreDistribution.jar       \
   I=/output/filename.b37_1kg.sorted.bam                                 \
   O=/output/filename.b37_1kg.[wiki:QualityScoreDistribution]            \
   CHART=/output/filename.b37_1kg.[wiki:QualityScoreDistribution].pdf    \
   VALIDATION_STRINGENCY=LENIENT                                        \
   TMP_DIR=/local

BamIndexStats

java -jar /tools/picard-tools-1.32/BamIndexStats.jar \
   INPUT=/output/filename.b37_1kg.sorted.bam          \
   VALIDATION_STRINGENCY=LENIENT                     \
   TMP_DIR=/local

CalculateHsMetricsWholeGenome

java -jar -Xmx3g /tools/picard-tools-1.32/CalculateHsMetricsWholeGenome.jar \
   INPUT=/output/filename.b37_1kg.sorted.bam                                 \
   OUTPUT=/output/filename.b37_1kg.HsMetrics                                 \
   BAIT_INTERVALS=/resources//hg19/intervals/GoNL.interval_list             \
   TARGET_INTERVALS=/resources//hg19/intervals/GoNL.interval_list           \
   VALIDATION_STRINGENCY=LENIENT                                            \
   TMP_DIR=/local

Split BAMS by Chromosome

BAMs are split into chromosomes BAMs. These files move on to variant calling.

Compress BAMS with ReduceRead

Variant Calling

UnifiedGenotyper

java -jar GenomeAnalysisTK.jar \
  -R resources/Homo_sapiens_assembly18.fasta \
  -T UnifiedGenotyper \
  -I sample1.bam [-I sample2.bam ...] \
  --dbsnp dbSNP.vcf \
  -o snps.raw.vcf \
  -stand_call_conf [50.0] \
  -stand_emit_conf 10.0 \
  -dcov [50 for 4x, 200 for >30x WGS or Whole exome] \
  [-L targets.interval_list]


VariantRecalibrator

java -Xmx4g -jar GenomeAnalysisTK.jar \
  -T VariantRecalibrator \
  -R reference/human_g1k_v37.fasta \
  -input NA12878.HiSeq.WGS.bwa.cleaned.raw.subset.b37.vcf \
  -resource:hapmap,known=false,training=true,truth=true,prior=15.0 hapmap_3.3.b37.sites.vcf \
  -resource:omni,known=false,training=true,truth=false,prior=12.0 1000G_omni2.5.b37.sites.vcf \
  -resource:dbsnp,known=true,training=false,truth=false,prior=6.0 dbsnp_135.b37.vcf \
  -an QD -an HaplotypeScore -an MQRankSum -an ReadPosRankSum -an FS -an MQ -an InbreedingCoeff \
  -mode SNP \
  -recalFile path/to/output.recal \
  -tranchesFile path/to/output.tranches \
  -rscriptFile path/to/output.plots.R

ApplyRecalibration

java -Xmx3g -jar GenomeAnalysisTK.jar \
  -T ApplyRecalibration \
  -R reference/human_g1k_v37.fasta \
  -input NA12878.HiSeq.WGS.bwa.cleaned.raw.subset.b37.vcf \
  --ts_filter_level 99.0 \
  -tranchesFile path/to/output.tranches \
  -recalFile path/to/output.recal \
  -mode SNP \
  -o path/to/output.recalibrated.filtered.vcf

Variant File Analyses

Family Based Analyses

IBD/SGS

Linkage

Population Based Analyses

Phasing

FST

Phenotype Based Analyses

GWAS

VAAST