Comparing annotations from different annotation pipelines--UNDER CONSTRUCTION

This page will contain instructions for for using MAKER to compare annotations generated by different pipelines.

As annotation becomes more tractable for small communities it is not uncommon for an organism to be annotated multiple times. Often these annotations are produced by different groups, pipelines, evidence sets, and practices. These differences make an apples to apples comparison challenging at best. Further complicating this is the lack of quality metrics assigned to annotated features by many pipelines. MAKER provides means for comparing such annotations.

MAKER assigns quality metrics based on the evidence to all of the protein coding gene annotations it produces. These quality metrics are discussed further down in this page. You may be wondering how this is going to help in comparing annotations from pipelines other than MAKER. Well you can pass the annotations from any pipeline in GFF3 and MAKER will calculate the quality metrics on those annotations for you. Now you can compare annotation sets from any pipeline.